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KMID : 0613820190290060637
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Journal of Life Science
2019 Volume.29 No. 6 p.637 ~ p.644
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Genomic Polymorphism Analysis Using Microsatellites in the Jeju Dogs
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Ko Min-Jeong
Kwon Seul-Gi
Kim Hye-Ran
Byun Jae-Hyun
Kim Dae-Cheol
Choi Bong-Hwan
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Abstract
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This study was conducted to analyze the genetic characteristics of the Jeju dog for preservation and protection. A total of 139 dogs from 7 dog breeds, including the Jeju dog, were genotyped using 16 microsatellite markers. The results revealed 2-18 alleles per locus, with a total of 131 alleles among the 16 markers. Most alleles were identified for FH3381, which had 18 alleles, whereas FH2834 had the fewest alleles, with just 2. When the total mean value was observed, the expected heterozygosity and observed heterozygosity were higher for than for outgroup dogs, and the PIC values ranged from 0.000 to 0.862, respectively. The phylogenetic tree analysis of the Jeju dog and other dog varieties revealed that the Jeju dog is closest to the Sapsal dog (0.393). The phylogeny between the Jeju and Korean domestic dogs showed that the Jeju dog is most distant from the Dongkyung dog (0.507). Looking at the distribution individually, the Jeju dog is in the same group as the Labrador Retriever and the Sapsal dog. Meanwhile, the Poongsan, Dongkyung, and Jindo dogs and the German Shepherd were in the same group. Genetic information confirmed through the results of this study can be used as basic data to study the genetic characteristics of the Jeju dog.
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KEYWORD
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Heterozygosity, Jeju dog, microsatellite markers, multiplex PCR
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